Cell Body-Motor Neuron Counts.pdf

sma-SMA_M.1.2.004.pdf
Preview of Cell Body-Motor Neuron Counts
🔗 Source: treat-nmd.org
📊 Size: 857 KB
👤 Author: Raffa
⬇️ Downloads: 274

Summary

SMA_M.1.2.004 is a Standard Operating Procedure (SOP) for cell body-motor neuron counts in the SMA mouse model, describing methods and reference values for quantitative and reliable measurement of pathology-relevant histological parameters. The SOP includes two protocols: Haematoxylin and Eosin (H&E) or thionine staining, and choline acetyl-transferase (ChAT) staining. The procedure requires careful surgical dissection of spinal cord, cryosectioning, and staining, with specific materials and working solutions listed. The methods involve termination under protocol, cryosectioning, H&E staining, ChAT staining, and thionine staining, with evaluation and interpretation of results.

Description

SMA_M.1.2.004 is a Standard Operating Procedure (SOP) for cell body-motor neuron counts in the SMA mouse model, describing methods and reference values for...

Technical Information

  • File Format: PDF
  • File Size: 857 KB
  • Pages: 13
  • Language: EN
  • Author: Raffa
  • Total Downloads: 274
  • Last Updated: 1 month ago

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