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Green Version.pdf

10.1007s10577-014-9459-z.pdf
Preview of Green Version
🔗 Source: link.springer.com
📊 Size: 1.13 MB
📄 Pages: 23 pages
⬇️ Downloads: 95

Summary

Microcell-mediated chromosome transfer (MMCT) is a technique that transfers a chromosome from donor cells to recipient cells, used for gene delivery and somatic cell genetics. However, transferring an entire chromosome or fragment is difficult for studying a single specific gene. Human artificial chromosomes (HACs) and mouse artificial chromosomes (MACs) are alternative tools that can carry a gene or genes of interest. HACs/MACs have been generated through top-down or bottom-up approaches and exhibit characteristics of ideal gene delivery vectors, including stable episomal maintenance and the capacity to carry large genomic loci. MMCT is used to manipulate HACs and MACs in donor cells and deliver them to recipient cells, allowing for exogenous gene expression in cultured cells and transgenic animals. This technique has various applications, including gene therapy, and proposes new avenues for addressing bio-medical challenges.

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Description

Microcell-mediated chromosome transfer (MMCT) is a technique that transfers a chromosome from donor cells to recipient cells, used for gene delivery and...

Technical Information

  • File Format: PDF
  • File Size: 1.13 MB
  • Pages: 23
  • Language: EN
  • Total Downloads: 95
  • Last Updated: 3 weeks ago

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