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Summary

A431 cells were treated with pervanadate, a protein tyrosine phosphatase inhibitor, to increase tyrosine phosphorylation. The cells were serum starved, then either left untreated or treated with 1 mM pervanadate for 30 minutes at 37°C. The resulting lysate is supplied at 1 mg/ml in electrophoresis sample buffer and can be used for western blot analysis to detect tyrosine phosphorylation.

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A431 cells were treated with pervanadate, a protein tyrosine phosphatase inhibitor, to increase tyrosine phosphorylation.

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