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LAH #246-01.pdf

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🔗 Source: herzenberglab.stanford.edu

📊 Size: 2.9 MB

📄 Pages: 45 pages

⬇️ Downloads: 66

Summary

Fluorescence-activated cell sorting (FACS) is a technique used for the analysis and separation of cell populations. It allows for quantitative, multiparameter measurements on individual cells, making it possible to evaluate specific cell populations even in complex mixtures. FACS has been aided by the development of monoclonal antibodies, which have increased the range and effectiveness of immunofluorescence measurements. The technique has a wide range of applications, including basic and clinical research, and is coming into use for clinical diagnostic purposes. However, FACS has limitations, such as a limited cell flow rate of about 2000 cells/sec, which can make it difficult to sort large numbers of cells in a reasonable time. In comparison, free-flow electrophoresis can separate cells at a rate of 100,000 cells/sec, making it a more efficient method for certain applications.

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Description

Fluorescence-activated cell sorting (FACS) is a technique used for the analysis and separation of cell populations.

Technical Information

File Format: PDF
File Size: 2.9 MB
Pages: 45
Language: EN
Total Downloads: 66
Last Updated: 7 days ago

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