LAH #453.pdf

lah453-bigos.pdf
Preview of LAH #453
🔗 Source: herzenberglab.stanford.edu
📊 Size: 288 KB
📄 Pages: 10 pages
⬇️ Downloads: 213

Summary

Nine color eleven parameter immunophenotyping using three laser flow cytometry allows for the simultaneous evaluation of nine distinct fluorescent parameters on one cell sample. The study compares the quality of data obtained with full software compensation and partial spectral compensation of selected pairs of parameters in analog hardware, combined with final software compensation. The use of partial analog compensation reduces variation in background staining levels introduced by the compensation process, enabling the characterization of novel B cell populations with frequencies below 1%. The choice of reagent-dye combinations and the ability to compensate in multi-parameter measurement space are crucial to obtaining satisfactory results.

Description

Nine color eleven parameter immunophenotyping using three laser flow cytometry allows for the simultaneous evaluation of nine distinct fluorescent parameters...

Technical Information

  • File Format: PDF
  • File Size: 288 KB
  • Pages: 10
  • Language: EN
  • Total Downloads: 213
  • Last Updated: 4 weeks ago

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