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DharmaFECT™ reverse transfec tion of siRNA pdf

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Reverse-transfection-of-sirna-protocol.pdf - one. RTF plates offer the advantage of predispensed siRNAs, while reverse transfection offers the flexibility of testing siRNA reagents at various concentrations or utilizing existing siRNA library resources in a standard transfection format. A. Standard

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Silencer_select_transfection_protocol_man0014605_ug.pdf - siRNA resuspension guidelines We recommend preparing 10 μM siRNA stock solutions. 1. Briefly centrifuge the plate to ensure that the dried siRNA is at the bottom of the wells. 2. Resuspend the siRNA in nuclease-free water at a final concentration of 10 ?

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