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Solvent Substitution Guide For C18 Reverse Phase Chromatography On CombiFlash Systems.pdf

Acetonitrile-Methanol Substitution in C-18 Reverse Phase App Note.pdf
Preview of Solvent Substitution Guide for C18 Reverse Phase Chromatography on CombiFlash Systems
🔗 Source: teledyneisco.com
📊 Size: 119 KB
👤 Author: Teledyne Isco, Inc.
⬇️ Downloads: 146

Summary

This application note provides a method to estimate the concentration of substitute solvents for reverse phase MPLC, particularly when switching from acetonitrile to other options due to cost or availability. The key points include:

- Solvent Conversion: A nomogram (Figure 1) assists in determining equivalent concentrations of various organic solvents commonly used in reverse phase chromatography. It allows for substitution while maintaining similar purification results.

- Isocratic Methods: Experiments using methyl and propyl paraben on celite demonstrate that isocratic runs are sensitive to solvent concentration. The nomogram provides a starting point for adjusting methanol and tetrahydrofuran (THF) concentrations (±5%) for optimal results.

- Gradient Purification: For gradient methods, the nomogram guides the creation of alternative gradients, allowing for tailored selectivity based on compound classes.

- Applications: C18 methods can be easily adapted to alternative solvents, offering flexibility in purification strategies and cost savings. The solvent substitution chart is a valuable tool for method development.

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Description

Substituting acetonitrile or methanol in C18 reverse-phase chromatography on CombiFlash systems, this note provides a nomogram to determine equivalent concentrations for optimal purification when original solvents are unavailable or cost-prohibitive.

Technical Information

  • File Format: PDF
  • File Size: 119 KB
  • Pages: 2
  • Language: EN
  • Author: Teledyne Isco, Inc.
  • Total Downloads: 146
  • Last Updated: 5 days ago

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This PDF document about Solvent Substitution Guide for C18 Reverse Phase Chromatography on CombiFlash Systems provides comprehensive information and guidance. Whether you're a beginner or advanced user, this resource offers valuable insights into Solvent Substitution Guide for C18 Reverse Phase Chromatography on CombiFlash Systems.

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