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20567.pdf

20567.pdf
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Summary

Intravascular thrombosis causes cardiovascular diseases, and fibrinolytic agents are used to treat them. However, current agents have limitations, such as large therapeutic doses and bleeding complications. Microbial sources, including bacteria, fungi, and actinomycetes, can produce fibrinolytic enzymes. Pseudomonas putida B-18, isolated from soil, was found to produce a protease enzyme with fibrinolytic activity. The enzyme was produced using a soy meal wheat powder broth and purified using ammonium salt precipitation, dialysis, and DEAE cellulose. The enzyme's molecular weight was determined, and its activity was affected by temperature, pH, and metal ions. The enzyme showed in vitro blood clot lysis, making it a potential candidate for thrombolytic therapy.

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Intravascular thrombosis causes cardiovascular diseases, and fibrinolytic agents are used to treat them.

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  • File Format: PDF
  • File Size: 554 KB
  • Pages: 6
  • Language: EN
  • Total Downloads: 130
  • Last Updated: 1 week ago

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