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ATPase/GTPase Assay.pdf

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Preview of ATPase/GTPase Assay
🔗 Source: bioassaysys.com
📊 Size: 105 KB
👤 Author: BAS 310A
⬇️ Downloads: 244

Summary

The QuantiChrom ATPase/GTPase Assay Kit determines ATPase/GTPase activities in a microplate format with high sensitivity, detecting 0.007 U/L ATPase or GTPase activity. The kit features a single reagent for accurate determination of enzyme activity in 30 minutes at room temperature. Applications include determination of ATPase and GTPase activity and high-throughput screening for inhibitors. The kit contains 200 assays in a 96-well plate, with reagent, assay buffer, and standard. Storage conditions require the kit to be stored at 4°C, and precautions include handling the reagent with 0.27 M H2SO4. The assay procedure involves preparing phosphate standards, performing a series dilution of enzyme, and measuring OD620nm on a plate reader to calculate enzyme activity. Inhibitor assays and 384-well plate assays follow similar procedures.

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Description

The QuantiChrom ATPase/GTPase Assay Kit determines ATPase/GTPase activities in a microplate format with high sensitivity, detecting 0.007 U/L ATPase or GTPase...

Technical Information

  • File Format: PDF
  • File Size: 105 KB
  • Pages: 2
  • Language: EN
  • Author: BAS 310A
  • Total Downloads: 244
  • Last Updated: 1 day ago

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