Beta-lactamase Detection.pdf

TOKU-E Nitrocefin Beta-Lactamase Detection Protocol_Jan2023.pdf
Preview of Beta-lactamase Detection
🔗 Source: store-njqa715u56.mybigcommerce.com
📊 Size: 228 KB
👤 Author: Alice Ledbetter
⬇️ Downloads: 51

Summary

Nitrocefin is used to detect β-lactamases through chromogenic assays. A stock solution (10 mg/ml) is prepared in DMSO and then diluted to a working solution (0.5-1.0 mg/ml) in PBS. The working solution appears yellow and turns red upon exposure to β-lactamase. Various detection assays include slide surface, direct contact, broth suspension, lysed cell, and filter paper assays, all producing a positive result within 20-30 minutes. Spectrophotometric analysis measures β-lactamase activity based on Nitrocefin degradation.

Description

Nitrocefin is used to detect β-lactamases through chromogenic assays.

Technical Information

  • File Format: PDF
  • File Size: 228 KB
  • Pages: 3
  • Language: EN
  • Author: Alice Ledbetter
  • Total Downloads: 51
  • Last Updated: 1 month ago

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This PDF document about Beta-lactamase Detection provides comprehensive information and guidance. Whether you're a beginner or advanced user, this resource offers valuable insights into Beta-lactamase Detection.

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