Biological Conversion Of Sugipine Components From Sug Pine Leaves Using Strobilurus Ohshimae.pdf

This study investigates the potential of the edible mushroom Strobilurus ohshimae (Sugi Edatake) in transforming and utilizing杉 (Japanese cedar) needle leaves, a by-product of significant national production.

Method:

- S. ohshimae was used to cultivate and process samples of杉 needle leaves.
- 3 different tests were conducted:
- Testing the weight loss rate after placing S. ohshimae-cultivated branches in a controlled environment for 2, 4, and 6 months.
- Analyzing the composition changes of leaf powder after fungal treatment over the same time periods.
- Extracting and identifying compounds using GC/MS analysis.
- Controls included untreated samples and Gloeophyllum trabeum (Kichirimentake) for comparison.

Key Findings:

- Weight Loss: After 6 months, fungal treatment resulted in a 22% weight loss of the branches compared to controls.
- Composition Changes: While holocellulose and Klason lignin content changes were minimal, significant decreases were observed in extractable components (mainly (-)-kaurene) after 2 months of treatment.
- Extract Analysis: GC/MS revealed that untreated samples retained (-)-kaurene for up to 6 months, while treated samples showed a near complete absence of this compound after 2 months.

Implications:

The study suggests:

- Effective Composting: S. ohshimae can effectively reduce the weight and extractable components of杉 needle leaves, making them suitable for composting.
- Pharmaceutical Potential: The removal of (-)-kaurene, a valuable compound with agricultural and pharmaceutical uses, highlights the potential of S. ohshimae in producing bioactive compounds from needle leaves.
- Waste Utilization: Edible mushrooms like S. ohshimae offer a sustainable method for utilizing杉 needle leaf waste, contributing to circular economy practices in forestry.