Genetic Markers.pdf

The study utilized various molecular biology techniques, including genetic mapping, PCR, and sequencing, to investigate the cloche mutation. Key findings include:

Identification of SSLP markers and their respective PCR primer combinations used for genetic mapping (Supplementary Table 1).
Mapping of sequencing reads from total RNA and polyA enriched mRNA of clom39 mutants and wild-type siblings, which identified 13 genomic loci of interest with reduced expression in clom39 mutants (Supplementary Table 2).
Targeting of candidate genes using TALEN or CRISPR/Cas9 technology, with the TALEN arms or sgRNA guides listed in Supplementary Table 3.
Use of HRMA with specific primer combinations to identify mutant alleles.
* List of primers used in the study, including those for qPCR, sequencing, and in situ hybridization (Supplementary Table 4).

The study focused on the cloche mutation and its effects on gene expression, with a particular emphasis on the identification of candidate genes and the use of various molecular biology techniques to investigate the mutation. The findings provide insight into the genetic mechanisms underlying the cloche mutation and its impact on gene expression.