Moss Inc. Enzyme Substrates: Categories, Applications, And Signals.pdf

Moss-Substrates-Overview-Dec07.22.pdf
Preview of Moss Inc. Enzyme Substrates: Categories, Applications, and Signals
🔗 Source: mossbio.com
📊 Size: 1.28 MB
👤 Author: zdown
⬇️ Downloads: 44

Summary

Moss Inc. offers a range of enzyme substrates, primarily liquid mixtures of chemicals used in medical testing. These substrates are designed to interact with specific enzymes, causing a detectable change when combined. They fall into two main enzyme categories: Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP), and two application categories: soluble and insoluble.

Categorization:
- Enzyme Categories: HRP and AP.
- Application Categories: Soluble substrates for ELISA, microarray, and histochem applications; insoluble substrates for membrane-based assays.

Signal Categories:
- Chromogenic: Substrates that produce a color change upon enzyme action (e.g., ABTS, DAB).
- Luminogenic: Emitting light when enzymes act on them (e.g., TMBES, ChemiHRP).

Substrate Types:

Soluble Substrates:

- HRP:
- One-component: ABTS, TMBES, TMBE, TMBUS, TMBHK.
- Two-component: DAB, ChemiHRP (requiring a mediator).

- AP:
- One-component: NPPD.
- Two-component: PNPP (50x) + PNPB.

Insoluble Substrates:

- Used in membrane-based assays for both HRP and AP enzymes, employing various detection methods.

Description

Moss Inc. Enzyme Substrates offer liquid chemical mixtures for medical testing, designed to interact with specific enzymes and induce a detectable change. Categorized by enzyme type (Horseradish Peroxidase or Alkaline Phosphatase) and application (soluble or insoluble), they support various techniques including ELISA, histochemistry, and microarrays.

Technical Information

  • File Format: PDF
  • File Size: 1.28 MB
  • Pages: 14
  • Language: EN
  • Author: zdown
  • Total Downloads: 44
  • Last Updated: 3 weeks ago

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