Protocol.pdf

KOA0622.pdf
Preview of Protocol
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📊 Size: 376 KB
👤 Author: Jahan Ara
⬇️ Downloads: 442

Summary

Mouse MMP-12 AccuSignalTM ELISA Kit is for quantitative detection of Mouse MMP-12 in cell culture supernatants, serum, and plasma. The kit includes pre-coated ELISA plate, target protein standard, biotinylated detection antibody, ABC complex, and other reagents. Store at 4˚C for 6 months or -20˚C for 12 months. The assay employs a monoclonal antibody specific for MMP-12 and a biotinylated detection polyclonal antibody. Required equipment includes microplate reader, automated plate washer, and adjustable pipettes. Sample preparation involves storing samples at 2-8°C, centrifuging, and aliquoting. Sample dilution factors must be determined by the investigator. The kit provides a standard curve with 8 points, ranging from 0 to 4000 pg/mL. Reconstitution of the Mouse MMP-12 standard and preparation of standard curve are described in the protocol.

Description

Mouse MMP-12 AccuSignalTM ELISA Kit is for quantitative detection of Mouse MMP-12 in cell culture supernatants, serum, and plasma.

Technical Information

  • File Format: PDF
  • File Size: 376 KB
  • Pages: 11
  • Language: EN
  • Author: Jahan Ara
  • Total Downloads: 442
  • Last Updated: 1 week ago

Document Overview

This PDF document about Protocol provides comprehensive information and guidance. Whether you're a beginner or advanced user, this resource offers valuable insights into Protocol.

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