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Static.igem.org/medi Awiki/2018/d/dd/T--R Ice--June.pdf

T--Rice--June.pdf
Preview of static.igem.org/medi awiki/2018/d/dd/T--R ice--June.pdf
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📊 Size: 78.03 MB
📄 Pages: 53 pages
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Summary

Researchers conducted experiments from June 3rd to June 7th, 2018, at Keck 201 and ABL 126. The goals included creating competent cells, miniprep, and making new plates. On Monday, they made TSS buffer, created competent cells using DH5a cells, and performed DNA miniprep using a QIAGEN protocol. On Tuesday, they remade TSS buffer, prepped DNA, and checked the OD of liquid cultures. They also performed heat shock and plated bacteria on agar plates, but observed overgrowth on two plates and no growth on the other two. On Wednesday, they made new agar plates with chloramphenicol and performed an antibiotic comparison test. On Thursday, they stored the new plates, checked the antibiotic comparison plate, and performed NanoDrop measurements on H.W. samples. The antibiotic comparison test showed that the antibiotic added to the original plates was not effective.

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Description

Researchers conducted experiments from June 3rd to June 7th, 2018, at Keck 201 and ABL 126.

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  • File Format: PDF
  • File Size: 78.03 MB
  • Pages: 53
  • Language: EN
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  • Last Updated: 6 hours ago

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