TR-FRET Immunoassay.pdf

AN_P33_FRET_ForWeb.pdf
Preview of TR-FRET Immunoassay
🔗 Source: edinst.com
📊 Size: 454 KB
📄 Pages: 2 pages
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Summary

Förster resonance energy transfer (FRET) is a phenomenon of non-radiative transfer of optical excitation from a donor to an acceptor molecule, used to measure separating distance between molecules. Lanthanides like Europium Cryptate have long emission lifetimes and high transfer efficiency to acceptors like Allophycocyanin. Time-Resolved FRET (TR-FRET) measures donor and acceptor intensities in "gated" mode, but has drawbacks like emission spectrum overlap and requirement of two detectors. A time-resolved fluorescence format can increase sensitivity and reduce cost by applying multi-channel scaling mode to measure donor emission time-responses, allowing separation of microsecond background and avoiding spectral "cross-talk".

Description

Förster resonance energy transfer (FRET) is a phenomenon of non-radiative transfer of optical excitation from a donor to an acceptor molecule, used to measure...

Technical Information

  • File Format: PDF
  • File Size: 454 KB
  • Pages: 2
  • Language: EN
  • Total Downloads: 102
  • Last Updated: 4 weeks ago

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