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HUYGENS PROFESSIONAL APPLICATION GUIDE

Microscope Type: Widefield / Confocal / Nipkov Disc
Objective Lens Numerical Aperture:
10X: 0.3, 20X: 0.5, 40X: 1.3 (Oil), 63X: 1.4 (Oil), 63X: 1.2 (Water)
Lens Medium Refractive Index: 1.518 (Zeiss Oil N518), 1.33 (Water), 1.0008 (Air)
Medium Refractive Index: 1.32 (Water), 1.44 (Glycerol 75%), 1.0008 (Air)
Excitation & Emission Wavelength: See experimental conditions
Pinhole radius: Calculated using backprojected pinhole radius (rb) formula
XYZ-Sample Size: See experimental conditions
Signal to Noise Ratio (SNR): Estimated using rules of thumb or calculated from pixel value of a single photon event
Alternative Method for SNR-estimation: Involves obtaining a representative image, generating a histogram, and calculating SNR using parameters such as background intensity and noise intensity.

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HUYGENS PROFESSIONAL APPLICATION GUIDE

Microscope Type: Widefield / Confocal / Nipkov Disc
Objective Lens Numerical Aperture:
10X: 0.3, 20X: 0.5, 40X: 1.3...

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  • File Format: PDF
  • File Size: 929 KB
  • Pages: 34
  • Language: EN
  • Total Downloads: 772
  • Last Updated: 3 hours ago

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