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K-AMYL Procedures.pdf

K-AMYL_DATA.pdf
Preview of K-AMYL Procedures
🔗 Source: megazyme.com
📊 Size: 517 KB
👤 Author: Adriana Quadrelli
⬇️ Downloads: 62

Summary

The K-AMYL kit measures amylose and amylopectin content in starch samples. Amylose is determined by enzymatically hydrolyzing it to D-glucose, which is then analyzed using glucose oxidase/peroxidase reagent. The kit uses concanavalin A (Con A) to specifically precipitate amylopectin, allowing for accurate measurement of amylose. The procedure involves dispersing starch samples in dimethyl sulfoxide, removing lipids with ethanol, and then precipitating amylopectin with Con A. The kit is suitable for 100 assays and contains various reagents, including Con A, amyloglucosidase, and glucose oxidase/peroxidase. The accuracy of the kit is high, with relative standard deviations of <5% for pure starches and ~10% for cereal flours.

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Description

The K-AMYL kit measures amylose and amylopectin content in starch samples.

Technical Information

  • File Format: PDF
  • File Size: 517 KB
  • Pages: 12
  • Language: EN
  • Author: Adriana Quadrelli
  • Total Downloads: 62
  • Last Updated: 6 days ago

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This PDF document about K-AMYL Procedures provides comprehensive information and guidance. Whether you're a beginner or advanced user, this resource offers valuable insights into K-AMYL Procedures.

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