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Letöltés.pdf

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Preview of Letöltés
🔗 Source: jasco.hu
📊 Size: 243 KB
👤 Author: JASCO International
⬇️ Downloads: 236

Summary

Aflatoxins, produced by microorganisms like Aspergillus flavus, have strong carcinogenic effects and are regulated by the Japanese Government, with a limit of 10 μg/kg for total aflatoxins. The Japanese Government strengthened regulations in 2011, and a new procedure using trifluoroacetic acid (TFA) derivatization and HPLC with a fluorescence detector was recommended to improve sensitivity. This application note applies the new procedure to both conventional HPLC and UHPLC, using a mixture of aflatoxin B1, B2, G1, and G2 as standard samples. TFA derivatization improves the fluorescence intensity of B1 and G1, and multi-functional or immunoaffinity columns are used for sample preparation to improve reproducibility and recovery. Corn grits and roasted peanuts were used as actual samples, with different preparation procedures applied to each. The results demonstrate the effectiveness of the new procedure for analyzing total aflatoxins in food.

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Description

Aflatoxins, produced by microorganisms like Aspergillus flavus, have strong carcinogenic effects and are regulated by the Japanese Government, with a limit of...

Technical Information

  • File Format: PDF
  • File Size: 243 KB
  • Pages: 5
  • Language: EN
  • Author: JASCO International
  • Total Downloads: 236
  • Last Updated: 14 hours ago

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