Thermo Scientific OTP Brochure.pdf

The Thermo Scientific Dharmacon ON-TARGETplus siRNA is the global market leader in terms of specificity for small interfering RNA (siRNA). It offers a comprehensive solution to reduce off-target effects, which can lead to toxicity or false phenotypes. Key features include:

- Dual-Strand Modification Pattern: Unique pattern designed to minimize off-targets caused by either siRNA strand.
- SMARTpool Technology: Pooled siRNAs that enhance effectiveness and decrease off-targets.
- Stringent Design Filters: Utilizes seed region filters and seed frequency analysis to minimize off-target effects.

Benefits:

- Reduces Off-Targets Up to 90%: Superior to unmodified or sense strand-inactivated siRNAs.
- Combines Specificity and Potency: Ensures guaranteed silencing with fewer false positives.
- Improves Confidence in RNAi Results: Eliminates uncertainty caused by off-target effects.

Proven Performance:

- Microarray Analysis: Demonstrated significant reduction in off-target genes (from 120 to 0) compared to unmodified and sense strand-inactivated siRNAs.
- Seed Region Optimization: Low seed frequency siRNAs have significantly fewer off-targets than medium or high frequency seeds.
- SMARTpool Efficacy: ON-TARGETplus SMARTpools eliminate false phenotypes while maintaining potent target gene knockdown.

Applications:

- Ideal for studying gene function and validation in various research and therapeutic settings.
- Ensures reliable and reproducible RNAi results, crucial for advanced biological investigations.