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AspFlex Protocols.pdf

aspflex_kit_description_rev.pdf
Preview of AspFlex Protocols
🔗 Source: media.addgene.org
📊 Size: 256 KB
👤 Author: Merlin Brychcy
⬇️ Downloads: 86

Summary

AspFlex reactions are similar to EcoFlex and protocols can be transferred, with adjusted protocols shown for optimal cloning efficiency. The cloning of a transcriptional reporter and a CRISPRi construct are described in detail, involving Golden Gate reactions and transformation into E. coli. The process involves designing primers, amplifying promoter regions, and assembling level 0 and level 1 plasmids, with subsequent verification via sequencing and screening. CRISPRi constructs can be combined with transcriptional reporters, and the kit provides plasmids for inducing dCas9 expression. Positive clones are confirmed via sequencing, and correct plasmids are introduced into A. baumannii for CRISPRi induction and verification.

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Description

AspFlex reactions are similar to EcoFlex and protocols can be transferred, with adjusted protocols shown for optimal cloning efficiency.

Technical Information

  • File Format: PDF
  • File Size: 256 KB
  • Pages: 3
  • Language: EN
  • Author: Merlin Brychcy
  • Total Downloads: 86
  • Last Updated: 3 weeks ago

Document Overview

This PDF document about AspFlex Protocols provides comprehensive information and guidance. Whether you're a beginner or advanced user, this resource offers valuable insights into AspFlex Protocols.

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AspFlex guideAspFlex tutorialProtocols guideProtocols tutorial

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