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ELISA Protocol For WSMoV.pdf

ELISA-protocol-for-WSMoV.pdf
Preview of ELISA protocol for WSMoV
🔗 Source: agconasia.com
📊 Size: 60 KB
👤 Author: Tom Burns
⬇️ Downloads: 336

Summary

ELISA protocol for WSMoV detection involves 10 steps:
1. Plan the ELISA plate layout with necessary controls and buffers, using duplicate wells.
2. Prepare 0.4g leaf samples in labelled bags, adding 800µl coating buffer, and grind the material.
3. Transfer samples to labelled eppendorf tubes, keeping them on ice or at 4°C.
4. Coat the plates with 100µl of each sample, incubating at 37°C for 1 hour.
5. Wash the plates with PBS-T and prepare the WSMoV antibody.
6. Add 100µl of diluted WSMoV antibody to each well, incubating at 37°C for 1 hour.
7. Wash the plates again and prepare the secondary conjugated antibody.
8. Add 100µl of diluted antibody to each well, incubating at 37°C for 1 hour.
9. Wash the plates and add 100µl of PNPP substrate to each well, allowing the color to develop.
10. Stop the reaction with 50µl of 3M NaOH and read the plate by eye or using an ELISA plate reader.

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Description

ELISA protocol for WSMoV detection involves 10 steps:
1. Plan the ELISA plate layout with necessary controls and buffers, using duplicate wells.
2.

Technical Information

  • File Format: PDF
  • File Size: 60 KB
  • Pages: 4
  • Language: EN
  • Author: Tom Burns
  • Total Downloads: 336
  • Last Updated: 19 hours ago

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This PDF document about ELISA protocol for WSMoV provides comprehensive information and guidance. Whether you're a beginner or advanced user, this resource offers valuable insights into ELISA protocol for WSMoV.

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ELISA guideELISA tutorialprotocol guideprotocol tutorialWSMoV guideWSMoV tutorial

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