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Ribotyping Protocol.pdf

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Preview of Ribotyping Protocol
🔗 Source: tceq.texas.gov
📊 Size: 32 KB
👤 Author: TCEQ
⬇️ Downloads: 125

Summary

Genomic DNA is isolated and restricted with EcoR1 and PvuII, then separated by gel electrophoresis and transferred to a Nitran filter. The filter is probed with 32P-labeled E. coli rRNA and hybridized overnight. After washing, the blots are exposed to x-ray film. Restriction fragment length polymorphism analysis is performed by assigning an alphanumeric pattern to each unique banding pattern, with identical numerical patterns but different banding patterns differentiated by letters. Ribotypes are entered into a database and compared to known sources, with isolates having identical PvuII and EcoR1 ribotypes deemed members of the same ribogroup.

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Description

Genomic DNA is isolated and restricted with EcoR1 and PvuII, then separated by gel electrophoresis and transferred to a Nitran filter.

Technical Information

  • File Format: PDF
  • File Size: 32 KB
  • Pages: 4
  • Language: EN
  • Author: TCEQ
  • Total Downloads: 125
  • Last Updated: 2 hours ago

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