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Viral Vector Production.pdf

improve-viral-vector-production-from-hek293-cells.original.pdf

🔗 Source: polyplus-sartorius.com

📊 Size: 443 KB

📄 Pages: 4 pages

⬇️ Downloads: 172

Summary

FreeStyleTM F17 and BalanCD® HEK293. No adaptation was required for cells to grow in BalanCD® HEK293, with similar viability and doubling times. Cell clumping was reduced in BalanCD® HEK293, resulting in better accessibility to transfection. Viral vector production was 40-50% higher in BalanCD® HEK293, with increased yields of lentivirus and adeno-associated virus (AAV) vectors. The study used a 2L benchtop bioreactor, with plans to scale up to a 200L stirred-tank bioreactor. Key findings include:

No adaptation required for HEK293 cells to grow in BalanCD® HEK293
Reduced cell clumping in BalanCD® HEK293
40-50% increase in viral vector production in BalanCD® HEK293
Improved yields of lentivirus and AAV vectors, including self-complementary genome construct scAAV9-SMN1.

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Description

HEK293 cells produce viral vectors for gene therapy. A new medium, BalanCD HEK293, improves yields of adeno-associated virus (AAV) and lentivirus (LV). It outperforms the current FreeStyle F17 medium.

Technical Information

File Format: PDF
File Size: 443 KB
Pages: 4
Language: EN
Total Downloads: 172
Last Updated: 1 week ago

Document Overview

This PDF document about Viral Vector Production provides comprehensive information and guidance. Whether you're a beginner or advanced user, this resource offers valuable insights into Viral Vector Production.