Nuclease Inactivation With Proteinase K General Protocol For Inactivating Nucleases With Proteinase K During The Extraction Of N.pdf

Nuclease Inactivation with Proteinase K.pdf
Preview of Nuclease Inactivation with Proteinase K General protocol for inactivating nucleases with proteinase K during the extraction of n
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Summary

Protocol for Nucleic Acids Extraction using Proteinase K:
Proteinase K inactivates nucleases that degrade DNA and RNA after cell lysis by digesting them.
Materials needed include Proteinase K and other extraction protocol materials.
The method involves cell lysis, adding Proteinase K to the lysate, incubating at 55°C for 1-3 hours, and then extracting the desired nucleic acid.
Proteinase K can be inactivated by heating to 95°C for 10 minutes after digestion.
The final concentration of Proteinase K should be 50-400 μg/ml.
References include Hilz et al. (1975), Goldenberger et al. (1995), McGookin (1984), and Maloy (1990).

Description

Protocol for Nucleic Acids Extraction using Proteinase K:
Proteinase K inactivates nucleases that degrade DNA and RNA after cell lysis by digesting them.

Technical Information

  • File Format: PDF
  • File Size: 107 KB
  • Pages: 2
  • Language: EN
  • Author: Content Writer
  • Total Downloads: 86
  • Last Updated: 4 weeks ago

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