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Supplementary Information PDF (2190K).pdf

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Preview of Supplementary information PDF (2190K)
🔗 Source: rsc.org
📊 Size: 2.09 MB
👤 Author: Gjonaj, L. (CCB)
⬇️ Downloads: 80

Summary

Lorina Gjonaj et al. combined tools to achieve selectivity for USP7, using FoldX for computational predictions and synthesizing Rh-ubiquitin mutants. They performed labelling experiments, Western-blotting, and pull-down experiments to analyze USP7 and other DUBs. The study utilized HAP1 cells and USP7KO HAP1 cells, and involved the use of various software, including FoldX and UCSF Chimera. The researchers synthesized activity-based probes and used preparative HPLC for purification, ultimately aiming to understand USP7 selectivity.

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Description

Lorina Gjonaj et al. combined tools to achieve selectivity for USP7, using FoldX for computational predictions and synthesizing Rh-ubiquitin mutants.

Technical Information

  • File Format: PDF
  • File Size: 2.09 MB
  • Pages: 24
  • Language: EN
  • Author: Gjonaj, L. (CCB)
  • Total Downloads: 80
  • Last Updated: 1 month ago

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