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DNA Preparation Protocol.pdf

13073_2018_607_MOESM1_ESM.pdf
Preview of DNA Preparation Protocol
🔗 Source: static-content.springer.com
📊 Size: 233 KB
👤 Author: Stewart, Douglas (NIH/NCI) [E]
⬇️ Downloads: 422

Summary

DNA was purified and libraries prepared using KAPA HyperPlus Kit and Bioo Scientific NEXTflex DNA Barcoded Adapters. Libraries were amplified by ligation-mediated PCR, then subjected to liquid phase sequence capture using NimbleGen's SeqCap EZ Human Exome Library. Captured DNA was amplified again by ligation-mediated PCR, then sequenced. Reads were trimmed, aligned to the hg19 reference genome, and analyzed for variants using Genome Analysis Toolkit and other software. Variants were annotated using in-house custom software and public databases. Ethnicity-informative variation was used to match cases and controls.

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Description

DNA was purified and libraries prepared using KAPA HyperPlus Kit and Bioo Scientific NEXTflex DNA Barcoded Adapters.

Technical Information

  • File Format: PDF
  • File Size: 233 KB
  • Pages: 6
  • Language: EN
  • Author: Stewart, Douglas (NIH/NCI) [E]
  • Total Downloads: 422
  • Last Updated: 16 hours ago

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This PDF document about DNA Preparation Protocol provides comprehensive information and guidance. Whether you're a beginner or advanced user, this resource offers valuable insights into DNA Preparation Protocol.

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