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Provisional PDF.pdf

1685607047PJZ_MH20221026041025-R3_Zhu et al.pdf

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📊 Size: 1.02 MB

📄 Pages: 6 pages

⬇️ Downloads: 73

Summary

A SYBR Green I real-time quantitative PCR (RT-qPCR) assay was established for the detection of Micropterus salmoides rhabdovirus (MSRV). The assay had a detection limit of 2.78×10^1 copies/μL, which was 100 times more sensitive than conventional PCR. The assay showed high sensitivity and specificity, with no cross-reaction with other aquatic viruses. Out of 104 clinical samples, 54 were detected positive by the RT-qPCR assay, while 22 were detected positive by conventional PCR. The method can provide reliable technical support for clinical diagnosis and epidemiological investigation of MSRV.

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Description

A SYBR Green I real-time quantitative PCR (RT-qPCR) assay was established for the detection of Micropterus salmoides rhabdovirus (MSRV).

Technical Information

File Format: PDF
File Size: 1.02 MB
Pages: 6
Language: EN
Total Downloads: 73
Last Updated: 1 week ago

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